Journal: Journal of visualized experiments : JoVE

Article Title: Direct-Coupled Electroretinogram (DC-ERG) for Recording the Light-Evoked Electrical Responses of the Mouse Retinal Pigment Epithelium

doi: 10.3791/61491

Figure Lengend Snippet: Table of Materials

Article Snippet: Maintain at RT In-Line Filter Whatman 6722–5001 To protect vacuum pump from aerosols Low Noise Cable for Microelectrode Holders WPI Inc 5372 Suggested for improving the length and placement of the cables and electrode holder assemblies Magnetic Ball Joint WPI Inc 500871 For magnetically positioning the electrode holder assembly on the stage MatLab Mathworks MatLab: For editing the analysis software MatLab Curvefit Toolbox Mathworks Toolbox for MatLab (only required for editing the analysis software) MatLab Compiler Mathworks Toolbox for MatLab (only required for editing and re-releasing the analysis software) MatLab Runtime version 9.5 Mathworks R2018b (9.5) Required to run the analysis software: https://www.mathworks.com/products/compiler/matlab-runtime.html Microelectrode Holders (45 degrees) WPI Inc MEH345–15 For holding the capillaries Needle (25 ga) Covidien 8881250313 For filling the capillary tubes with HBSS Needle (ground) Electrode Rhythmlink 13mm - one elctrode Subdermal needle electrode (ground) for mouse (13mm long, 0.4mm diameter needle, 1.5m leadwire) Regulator/Power Conditioner Furman P-1800 Or equivalent to remove DC-offset from noise introduced through power line Syringe (12 mL) Monoject 1181200777 For filling the capillary tubes with HBSS T-clip Cole-Parmer 06852–20 For electrode holder assembly Vacuum Desiccator Bel-Art 420120000 Clear polycarbonate bottom & cover Pharmacological treatment Lubricant eye gel Alcon 0078–0429-47 Helps lubricate corneal surface and maintain electrical contact with capillary electrodes Phenylephrine Hydrochloride 2.5% Akorn 17478–201-15 Short acting mydriatic eye drops (for pupil dilation) Proparacaine Hydrochloride 0.5% Akorn 17478–263-12 Local anesthetic for ophthalmic instillation Tropicamide 0.5% Akorn 17478–101-12 Short acting mydriatic eye drops (for pupil dilation) Xylazine AnaSed sc-362949Rx Analgesic and muscle relaxant Zetamine (Ketamine HCl) VetOne 501072 Anesthetic for intramuscular injections Open in a separate window list-behavior=simple prefix-word= mark-type=none max-label-size=4 1.1.

Techniques: Convection, Software, Eye Drops

Journal: Journal of visualized experiments : JoVE

Article Title: Direct-Coupled Electroretinogram (DC-ERG) for Recording the Light-Evoked Electrical Responses of the Mouse Retinal Pigment Epithelium

doi: 10.3791/61491

Figure Lengend Snippet: Table of Materials

Article Snippet: Low Noise Cable for Microelectrode Holders , WPI Inc , 5372 , Suggested for improving the length and placement of the cables and electrode holder assemblies.

Techniques: Convection, Software, Eye Drops

Journal: Journal of visualized experiments : JoVE

Article Title: Direct-Coupled Electroretinogram (DC-ERG) for Recording the Light-Evoked Electrical Responses of the Mouse Retinal Pigment Epithelium

doi: 10.3791/61491

Figure Lengend Snippet: (A) Image of the completed circuit accomplished by lowering the recording (glass capillary microelectrodes), reference, and ground electrodes into the same bath solution. This configuration enables preliminary tests to be run (prior to anesthetizing the mouse) to evaluate the characteristic impedance, noise, and drift. Inset (upper left) showing a side-view schematic of the custom microelectrode holder stand. (B) Representative image of the Impedance Checking Mode showing the appropriate values for electrode impedances. The impedance in the Left and Right eye electrodes should be comparable, within 5 KΩ of each other (e.g., Left eye: 38.7 KΩ vs. Right eye: 40.36 KΩ). The impedance of the mouth reference electrode should be less than 1 KΩ, whereas the tail electrode should be around 2.5 KΩ. (C) Representative image of the preview trace (Step 4/6) is shown. Step 4 (Long Flash No Light) is selected as no light is delivered during the preview of this step. The traces should be low noise and may have a slight drift that gradually fades with time to baseline. Once the traces have achieved a constant drift in both channels and become relatively flat, the actual recording can begin. (D) Using Step 5/6 (Long Flash 10 cd 7 min) after 0.5 min of darkness, a light step of 10 cd/m2 is delivered to the mouse for 7 min followed by a return to darkness for 1.5 min. (E) Image of the export parameters used to convert the data to a *.csv file. This precise format is required to run the DC-ERG analysis software.

Article Snippet: Low Noise Cable for Microelectrode Holders , WPI Inc , 5372 , Suggested for improving the length and placement of the cables and electrode holder assemblies.

Techniques: Software